Highly variable effect of sonication to dislodge biofilm-embedded Staphylococcus epidermidis directly quantified by epifluorescence microscopy: an in vitro model study
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چکیده
منابع مشابه
Cadmium Modulates Biofilm Formation by Staphylococcus epidermidis
The aim of the study was to evaluate the effect of cadmium exposure on Staphylococcus epidermidis (ATCC 35984) biofilm formation. Bacteria were cultured in the absence or presence of different concentrations (0-50 µM) of cadmium. Biofilm formation and bacterial viability were assessed. Quantitative Real Time-PCR (qRT-PCR) was used to determine the mRNA expression of molecular markers of S. epid...
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Objective(s): Staphylococcus epidermidis is a common cause of medical device-associated infections due to biofilm formation, and its elimination is extremely challenging. Although rifampin efficacy against S. epidermidis biofilms has been confirmed, its use as a single agent may lead to resistance. As such, it is assumed that the combination of rifampin and N-acetylcysteine (NAC) could exert ad...
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conclusions allicin vapor, or some decomposition product of allicin, causes inhibition of bacterial growth and biofilm formation. parallel enzymatic studies confirmed this inhibitory effect and suggest that sulfhydryl enzymes are involved in biofilm formation in the strain studied. results an inhibition effect was observed both in the bacterial cultures and the enzymatic solutions, and the exte...
متن کاملDaptomycin rapidly penetrates a Staphylococcus epidermidis biofilm.
Fluorescently tagged daptomycin accessed the interior of Staphylococcus epidermidis biofilm cell clusters within minutes. The diffusion coefficient of daptomycin in the biofilm was 28% of its value in pure water. Daptomycin activity against staphylococci embedded in biofilms is unlikely to be limited by penetration of the antibiotic into the biofilm.
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ژورنال
عنوان ژورنال: Journal of Orthopaedic Surgery and Research
سال: 2020
ISSN: 1749-799X
DOI: 10.1186/s13018-020-02052-3